Description
Intended Uses: This human Antithrombin III antigen assay is intended for the quantitative determination of total Antithrombin III antigen in human plasma.
Principle of the Assay: Human Antithrombin III will bind to the capture antibody coated on the microtiter plate. Free and complexed Antithrombin III will react with the antibody on the plate. After appropriate washing steps, polyclonal anti-human Antithrombin III primary antibody binds to the captured protein Excess primary antibody is washed away and bound antibody, which is proportional to the total Antithrombin III present in the samples, is reacted with the secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared using dilutions of purified Antithrombin III and is measured along with the test samples. Color development is proportional to the concentration of Antithrombin III in the samples.
Background: Antithrombin III is a glycolsylated plasma serine protease inhibitor that forms a stoichiometric complex with coagulation cascade enzymes [1]. Antithrombin III inhibits alpha-Thrombin as well as Factor Xa, IXa, XIa and XIIa with heparin enhanced kinetics [2]. Type 1 Antithrombin deficiency is characterized by decreased plasma antigen levels of Antithrombin III [3]